Like many other crops sunflower also suffers from soil- born pathogens like the fungus Verticillium dahliae, which can cause up to 30% yield loss. The usual way in agriculture to control this fungus at present is to use variety resistance. However, as resistances have also been reported in these varieties, alternative and more sustainable strategies are needed. One way to control this pest is biofumigation. Biofumigation is defined as the suppression of soil pests and diseases resulting from hydrolysis products, i.e., toxic isothiocyanates (ITCs), released in the soil after incorporation of Glucosinolate (GSL)-containing plant tissue (Angus et al. 1994). These released hydrolysis products have an biocide effect on the fungus.
Suitable plants for this purpose are brassica species like black mustard, turnip rape and fodder radish. Rebekka F. and Leila Patzelt (UL) spend their 4-weeks internship in September 2020 at EcoMeteor to investigate the total glucosinolate content and profile in different brassica species. During their time at iDiv they learn how to isolate glucosinolate out of different plant organs and how to analyze them via HPLC-UV (Grosser and van Dam, 2012). Furthermore they will perform mass spectrometric analysis to structurally describe unknown candidates. This project is part of a cooperation with Neila Ait Kaci Ahmed from the French National Research Institute for Agriculture, Food and the Environment – INRAE
Sugarcane is one of the main sources of sugar and bioenergy worldwide and has a crucial impact on Brazilian economy. Breeding programs produces sugarcane genotypes with different agronomical features like enhanced production potential and resistance to biotic stresses. However, the resistance molecular mechanisms of most sugarcane genotypes are still unraveled. For this collaborative research between Centro de Cana (IAC, Ribeirão Preto-SP), São Paulo State University (Unesp, Jaboticabal-SP), and iDiv (Leipzig, DE), Dr. Andressa Bini and Prof. Guilherme Rossi brought samples of sugarcane genotypes exposed or not to different biotic stressors. Dr. Bini is investigating the molecular responses of sugarcane genotypes against leaf scald disease caused by Xanthomonas albilineans. Dr. Rossi is investigating how different genotypes respond to herbivory using insects with different feeding habits. The main goal of this collaborative research is to understand better the interactions of sugarcane genotypes with competitors as a form to develop sugarcane pest and disease management using natural plant resistance.
Volatile organic compounds (VOC´s) play an important role in interaction between plants, animals and their environment. Static and dynamic approaches to collect VOC´s in combination with gas chromatography-mass spectrometry analysis is a powerful tool to understand the composition, chemistry and the dynamic of these compounds. The successful application of this tool is based on a profound basic knowledge about the correct sampling strategy, the working principle of a mass spectrometer and the data analysis and interpretation.
To enable PhD students to use this powerfull approach EcoMetEoR organised a 3-day workshop including lectures, student talks and hands-on sessions. 14 Students from the iDiv konsortium and also from abroad discussed with PostDocs and PI´s of the university of Leipzig and Jena about the current challenges in trapping volatiles in different systems and how to analyze them via GC-MS.
For the third time in a row the EcoMetEoR platform and the members of the MIE group organized the introductory course on Eco-Metabolomics for PhD students from the 12th to the 16th of August. We are very pleased of the very high interest in the course, more than 12 PhD students from the iDiv consortium and abroad ( e.g. France, Serbia, Hungary) participated. During that week, the students learned about the correct experimental design, how to harvest and process the samples, the working principles of LCMS and GCMS and about the challenges considering data analysis and metabolite prediction. Next to the lectures given by PostDocs and PhD students of the MIE group and Nicole van Dam, the students had the chance to apply the learned content directly during hands on sessions. Especially the data analysis with XCMS and MetaboAnalyst was highly appreciated. The EcoMetEoR platform thanks all the lecturers and participants for such a great week!
*Grosser, K., and van Dam, N.M. (2017). A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC). J Vis Exp.
DFG Project Proposal was accepted!
The invasion of exotic earthworms often causes non-random changes in diversity above- (Craven et al. 2017) and belowground (Eisenhauer 2010, Ferlian et al. 2018), suggesting that earthworm invasion may alter how trait-mediated mechanisms impact ecosystem functioning. The EcoMeteor platform will support the group of Professor Nico Eisenhauer and perform the untargeted metabolomics analysis for profile comparison.
EcoMeteor and MIE welcome Sandra Boswinkel
Sandra Boswinkel is a Bachelor Student from Radboud Unversity in Nijmwegen. In the course of her bachelor thesis she´s investigating the effects of geographical location on insect performance by comparing the performance of inland versus coastal populations of solanum dulcamara when they are exposed to the Colorado potato beetle (Leptinotarsa decemlineata). Solanum dulcamara belongs as potato to the family of Solanacea and was therefore selected as model plant to investigate potato pests. Furthermore, previous research on coastal vs inland populations of solanum dulcamara showed that the coastal plants were less desired than inland plants what might be due to their higher glycoalkaloid content. During her research stay from the 04th to the 15th of March, Sandra analyzed the abundance of glycoalkaloids and phytohormons in the leaves of Solanum dulcamara collected at 8 different collecting points from the coast and in the inland of the Netherlands.
Dr. Hisham Abd El-lateaf from the National Research Centre in Cairo spends a 2-weeks research stay with the EcoMetEoR platform in iDiv.
In his current project he is studying the analysis and quantification of glucosinolates in seeds and meals of moringa oleifera and moringa peregrina.The aim of this project is to maximize the utilization of the moringa meal that remains after oil extraction. By Studying the effect of using different procedures for oil extraction including (hydraulic pressing, Screw pressing and extraction by solvent) from two species of moringa seeds grown in Egypt (moringa oleifera and moringa peregrina) on the content of their meals of glucosinolates qualitatively and quantitatively with emphasis on the biological activity of pure glucosinolates against different cancer cell lines. In addition the investigation also aims to study the effect of aqueous treatment of the cakes of the previous two species of moringa seeds on the content of their meals of glucosinolates.
Contact details: firstname.lastname@example.org
Dr. Aarti Gupta and PhD Dimitri Orine are visting the EcoMetEoR platform to analyze the content of chlorogenic acids and different glycoalcaloids in Solanum lycopersicum under different watering conditions and myccorhiza colonization.
Dimitri Orine is a PhD student from the University of Neuchâtel in Switzerland. He is involved in a European Innovative Training Network called “Microbe-induced Resistance to Agricultural Pests”.This project is focused on basic and applied research on context-dependency of microbe-induced resistance, mechanisms, and impacts on plant performance and other biocontrol organisms. The aim is to improve our ability to predict the effectiveness of microbe-induced resistance under different biotic and abiotic conditions.As a part of the project, he carries out fundamental research to investigate the effect of eco-climatic adaptation into tolerance-induction by microbes against herbivore pests.
Dr. Aarti Gupta is a Post-doctoral Fellow at Institute of Plant Genetics at the Polish Academy of Sciences in Poznan (Poland).Her current research involves understanding the molecular mechanisms of incompatible interaction between the Hypericumperforatum and the non-host pathogen Agrobacteriumtumefaciens. For the purpose, she is employing transcriptomic, biochemical and functional genomics tools. Here at iDiv, she´s attending a comprehensive hands-on training on metabolomes, glucosinolates, and phytohormones extraction and data analysis.
Google Scholar: https://scholar.google.pl/citations?user=-e8OfUgAAAAJ&hl=en
From the 27th to 31st of August 15 PhD Students and 1 PostDoc participated the introductory workshop "Introduction to Ecometabolomics for Ecologists". The week was filled with lectures, hands-on sessions and many discussions aboutthe application of metabolomics in ecological research. The participants learned about the design of a metabolomic experiment, how to extract the metabolites and collect volatile compounds and how to process and interpret the MS Data.
From the 16th to the 18th of October 2017, 34 experts from Ecology, Biochemistry and Bioinformatics joined together at the iDiv core centre to discuss the current challenges and pitfalls of applying Metabolomics in ecological studies. They discussed experimental designs, analytical platforms, peak detection algorithms, tested data processing workflows and multivariate statistical analyses. During the workshop, the participants also explored the different bioinformatic tools which are currently available for metabolomics data processing and identification.
As fruitful outcome of this international meeting the review "Current Challenges in Plant Ecometabolomics" has been published by 30 participants in the International Journal for Molecular Science as part of the Special Issue Metabolomics in the Plant Sciences 2017.
NEW video protocol on Glucosinolate Extraction & Analysis published in JoVE
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Two postdoctoral researcher positions at the German Centre for Integrative Biodiversity Research (iDiv) in Leipzig.
Katja Steinauer, PostDoc at the Netherlands Institute of Ecology (NIOO-KNAW), stays with us in June 2018 as a guest researcher using the facilities of the EcoMetEoR platform.
For the next months, Abigail Moreno-Pedraza (CINVESTAV, Mexico) and Diandro Ricardo Barilli (UNESP, Brazil) stay with us as guest researchers of the EcoMetEoR platform. Abigail will use her time in…
MIE and EcoMetEoR host Jiao Qu
Homepage of the European Metabolomics Training Coordination Group (EmTraG) - Overview about Metabolomics Training courses and Metabolomics Training Centres
Homepage of the Metabolomics Society - Useful information on upcoming events, Subscription for Metabolomics Newsletter, Jobs, Tutorials, Databases
Molecular Interaction Ecology group at iDiv - Molecular and chemical mechanisms governing interactions between plants and their biotic and abiotic environment.